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<title>Dulbecco’s Modified Eagle’s Medium</title>

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<h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">Dulbecco’s Modified Eagle’s Medium</span></h1>

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<div id="mw-content-text" class="mw-body-content mw-content-ltr" lang="de" dir="ltr"><div class="mw-content-ltr mw-parser-output" lang="de" dir="ltr"><p><b>Dulbecco's Modified Eagle's Medium</b>, kurz <b>DMEM</b>, ist ein standardisiertes <a href="Zellkulturmedium" title="Zellkulturmedium">Zellkulturmedium</a> mit breiter Verwendbarkeit für humane und verschiedene tierische Zellen. DMEM ist eine von <a href="Renato_Dulbecco" title="Renato Dulbecco">Renato Dulbecco</a> im Jahr 1959 publizierte modifizierte Variante des <i><a href="Eagle's_Minimal_Essential_Medium" class="mw-redirect" title="Eagle's Minimal Essential Medium">Eagle's Minimum Essential Medium</a> (EMEM)</i>, welches im Vergleich zu EMEM eine vierfache Konzentration an <a href="Aminos%C3%A4uren" title="Aminosäuren">Aminosäuren</a> und <a href="Vitamin" title="Vitamin">Vitaminen</a> enthält.<sup id="cite_ref-1" class="reference"><a href="#cite_note-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup> Heutzutage sind zahlreiche Versionen von DMEM mit leicht unterschiedlichen Zusammensetzungen kommerziell erhältlich, z. B. mit oder ohne <a href="Phenolrot" title="Phenolrot">Phenolrot</a> und <a href="Glutamin" title="Glutamin">Glutamin</a>, bzw. niedrigem (1000 mg/L) oder hohem (4500 mg/L) Gehalt an <a href="Glucose" title="Glucose">Glucose</a>, welche für verschiedenen Zellkultur-Anwendungen optimiert sind.

</p>

<div class="mw-heading mw-heading2"><h2 id="Zusammensetzung">Zusammensetzung</h2></div>

<caption>Verschiedene MEM-Derivate, in [mg/L]

</caption>

<th>Bestandteil

</th>

</th>

</th>

</th>

</th>

</th></tr>

<tr>

<td><a href="Glycin" title="Glycin">Glycin</a></td>

<td>50

</td>

<td>30

</td>

<td>30

</td></tr>

<tr>

<td><a href="L-Alanin" class="mw-redirect" title="L-Alanin"><small>L</small>-Alanin</a></td>

<td>25

</td>

<td>

</td>

<td>25

</td></tr>

<tr>

<td><a href="L-Arginin" class="mw-redirect" title="L-Arginin"><small>L</small>-Arginin</a> × HCl</td>

<td>126

</td>

<td>84

</td>

<td>84

</td></tr>

<tr>

<td><a href="L-Asparagin" class="mw-redirect" title="L-Asparagin"><small>L</small>-Asparagin</a> × H<sub>2</sub>O</td>

<td>50

</td>

<td>

</td>

<td>25

</td></tr>

<tr>

<td><a href="L-Asparagins%C3%A4ure" class="mw-redirect" title="L-Asparaginsäure"><small>L</small>-Asparaginsäure</a></td>

<td>30

</td>

<td>

</td>

<td>30

</td></tr>

<tr>

<td><a href="L-Cystein" class="mw-redirect" title="L-Cystein"><small>L</small>-Cystein</a> × HCl × H<sub>2</sub>O</td>

<td>100

</td>

<td>

</td>

<td>91,4

</td></tr>

<tr>

<td><a href="L-Cystin" class="mw-redirect" title="L-Cystin"><small>L</small>-Cystin</a> × 2 HCl</td>

<td>31

</td>

<td>63

</td></tr>

<tr>

<td><a href="L-Glutamins%C3%A4ure" class="mw-redirect" title="L-Glutaminsäure"><small>L</small>-Glutaminsäure</a></td>

<td>75

</td>

<td>

</td>

<td>75

</td></tr>

<tr>

<td><a href="L-Glutamin" class="mw-redirect" title="L-Glutamin"><small>L</small>-Glutamin</a></td>

<td>292

</td>

<td>584

</td>

<td>584

</td></tr>

<tr>

<td><a href="L-Histidin" class="mw-redirect" title="L-Histidin"><small>L</small>-Histidin</a></td>

<td>31

</td>

<td>42

</td>

<td>

</td></tr>

<tr>

<td><a href="L-Histidin" class="mw-redirect" title="L-Histidin"><small>L</small>-Histidin</a> × HCl × H<sub>2</sub>O</td>

<td>42

</td>

<td>

</td>

<td>42

</td></tr>

<tr>

<td><a href="L-Isoleucin" class="mw-redirect" title="L-Isoleucin"><small>L</small>-Isoleucin</a></td>

<td>52

</td>

<td>105

</td>

<td>105

</td></tr>

<tr>

<td><a href="L-Leucin" class="mw-redirect" title="L-Leucin"><small>L</small>-Leucin</a></td>

<td>52

</td>

<td>105

</td>

<td>105

</td></tr>

<tr>

<td><a href="L-Lysin" class="mw-redirect" title="L-Lysin"><small>L</small>-Lysin</a> × HCl</td>

<td>73

</td>

<td>146

</td>

<td>146

</td></tr>

<tr>

<td><a href="L-Methionin" class="mw-redirect" title="L-Methionin"><small>L</small>-Methionin</a></td>

<td>15

</td>

<td>30

</td>

<td>30

</td></tr>

<tr>

<td><a href="L-Phenylalanin" class="mw-redirect" title="L-Phenylalanin"><small>L</small>-Phenylalanin</a></td>

<td>32

</td>

<td>66

</td>

<td>66

</td></tr>

<tr>

<td><a href="L-Prolin" class="mw-redirect" title="L-Prolin"><small>L</small>-Prolin</a></td>

<td>40

</td>

<td>

</td>

<td>40

</td></tr>

<tr>

<td><a href="L-Serin" class="mw-redirect" title="L-Serin"><small>L</small>-Serin</a></td>

<td>25

</td>

<td>42

</td>

<td>42

</td></tr>

<tr>

<td><a href="L-Threonin" class="mw-redirect" title="L-Threonin"><small>L</small>-Threonin</a></td>

<td>48

</td>

<td>95

</td>

<td>95

</td></tr>

<tr>

<td><a href="L-Tryptophan" class="mw-redirect" title="L-Tryptophan"><small>L</small>-Tryptophan</a></td>

<td>10

</td>

<td>16

</td>

<td>16

</td></tr>

<tr>

<td><a href="L-Tyrosin" class="mw-redirect" title="L-Tyrosin"><small>L</small>-Tyrosin</a>, Dinatriumsalz × 2 H<sub>2</sub>O</td>

<td>52

</td>

<td>104

</td>

<td>104

</td></tr>

<tr>

<td><a href="L-Valin" class="mw-redirect" title="L-Valin"><small>L</small>-Valin</a></td>

<td>46

</td>

<td>94

</td>

<td>94

</td></tr>

<tr>

<td><a href="Ascorbins%C3%A4ure" title="Ascorbinsäure">Ascorbinsäure</a></td>

<td>50

</td>

<td>

</td>

<td>

</td></tr>

<tr>

<td><a href="Biotin" title="Biotin">Biotin</a></td>

<td>0,1

</td>

<td>

</td>

<td>0,013

</td></tr>

<tr>

<td><a href="Cholinchlorid" class="mw-redirect" title="Cholinchlorid">Cholinchlorid</a></td>

<td>1

</td>

<td>4

</td>

<td>4

</td></tr>

<tr>

<td><a href="D-Calciumpantothenat" class="mw-redirect" title="D-Calciumpantothenat"><small>D</small>-Calciumpantothenat</a></td>

<td>1

</td>

<td>4

</td>

<td>

</td></tr>

<tr>

<td><a href="Fols%C3%A4ure" title="Folsäure">Folsäure</a></td>

<td>1

</td>

<td>4

</td>

<td>4

</td></tr>

<tr>

<td><a href="Niacinamid" class="mw-redirect" title="Niacinamid">Niacinamid</a></td>

<td>1

</td>

<td>4

</td>

<td>4

</td></tr>

<tr>

<td><a href="Pyridoxal" title="Pyridoxal">Pyridoxal</a> × HCl</td>

<td>1

</td>

<td>4

</td>

<td>4

</td></tr>

<tr>

<td><a href="Riboflavin" title="Riboflavin">Riboflavin</a></td>

<td>0,1

</td>

<td>0,4

</td>

<td>0,4

</td></tr>

<tr>

<td><a href="Thiaminhydrochlorid" class="mw-redirect" title="Thiaminhydrochlorid">Thiaminhydrochlorid</a></td>

<td>1

</td>

<td>4

</td>

<td>4

</td></tr>

<tr>

<td><a href="Vitamin_B12" class="mw-redirect" title="Vitamin B12">Vitamin B<sub>12</sub></a></td>

<td>1,36

</td>

<td>

</td>

<td>0,013

</td></tr>

<tr>

<td><a href="I-Inositol" class="mw-redirect" title="I-Inositol">i-Inositol</a></td>

<td>2

</td>

<td>7,2

</td>

<td>7,2

</td></tr>

<tr>

<td><a href="Calciumchlorid" title="Calciumchlorid">Calciumchlorid</a> (CaCl<sub>2</sub>) (anhyd.)</td>

<td>200

</td>

<td>200

</td>

<td>165

</td></tr>

<tr>

<td><a href="Eisennitrat" class="mw-disambig" title="Eisennitrat">Eisennitrat</a> (Fe(NO<sub>3</sub>)<sub>3</sub> × 9 H<sub>2</sub>O)

</td>

<td>

</td>

<td>

</td>

<td>

</td>

<td>0,1

</td>

<td>

</td></tr>

<tr>

<td><a href="Kaliumnitrat" title="Kaliumnitrat">Kaliumnitrat</a></td>

<td>

</td>

<td>

</td>

<td>0,076

</td></tr>

<tr>

<td><a href="Kaliumchlorid" title="Kaliumchlorid">Kaliumchlorid</a> (KCl)</td>

<td>400

</td>

<td>400

</td>

<td>330

</td></tr>

<tr>

<td><a href="Magnesiumsulfat" title="Magnesiumsulfat">Magnesiumsulfat</a> (MgSO<sub>4</sub>) (anhyd.)</td>

<td>97,67

</td>

<td>97,67

</td>

<td>97,67

</td></tr>

<tr>

<td><a href="Natriumhydrogencarbonat" title="Natriumhydrogencarbonat">Natriumhydrogencarbonat</a> (NaHCO<sub>3</sub>)</td>

<td>2200

</td>

<td>3700

</td>

<td>3024

</td></tr>

<tr>

<td><a href="Natriumchlorid" title="Natriumchlorid">Natriumchlorid</a> (NaCl)</td>

<td>6800

</td>

<td>6400

</td>

<td>4505

</td></tr>

<tr>

<td><a href="Natriumdihydrogenphosphat" title="Natriumdihydrogenphosphat">Natriumdihydrogenphosphat</a> (NaH<sub>2</sub>PO<sub>4</sub> × H<sub>2</sub>O)</td>

<td>140

</td>

<td>125

</td>

<td>125

</td></tr>

<tr>

<td><a href="Natriumselenit" title="Natriumselenit">Natriumselenit</a></td>

<td>

</td>

<td>

</td>

<td>0,017

</td></tr>

<tr>

<td><a href="D-Glucose" class="mw-redirect" title="D-Glucose"><small>D</small>-Glucose</a> (Dextrose)</td>

<td>1000

</td>

<td>1000/4500

</td>

<td>4500

</td></tr>

<tr>

<td><a href="Lipons%C3%A4ure" title="Liponsäure">Liponsäure</a></td>

<td>0,2

</td>

<td>

</td>

<td>

</td></tr>

<tr>

<td><a href="Phenolrot" title="Phenolrot">Phenolrot</a></td>

<td>10

</td>

<td>15

</td>

<td>15

</td></tr>

<tr>

<td><a href="Natriumpyruvat" title="Natriumpyruvat">Natriumpyruvat</a></td>

<td>110

</td>

<td>110

</td>

<td>110

</td></tr>

<tr>

<td><a href="HEPES" title="HEPES">HEPES</a></td>

<td>

</td>

<td>

</td>

<td>5958

</td></tr></tbody></table>

<div class="mw-heading mw-heading2"><h2 id="Einzelnachweise">Einzelnachweise</h2></div>

<li id="cite_note-1"><span class="mw-cite-backlink"><a href="#cite_ref-1">↑</a></span> <span class="reference-text">R. Dulbecco, G. Freeman: <cite style="font-style:italic">Plaque production by the polyoma virus</cite>. In: <cite style="font-style:italic">Virology</cite>. <span style="white-space:nowrap">Band<span style="display:inline-block;width:.2em"> </span>8</span>, <span style="white-space:nowrap">Nr.<span style="display:inline-block;width:.2em"> </span>3</span>, 1. Juli 1959, <a href="Internationale_Standardnummer_f%C3%BCr_fortlaufende_Sammelwerke" title="Internationale Standardnummer für fortlaufende Sammelwerke">ISSN</a> <span style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://zdb-katalog.de/list.xhtml?t=iss%3D%220042-6822%22&key=cql">0042-6822</a></span>, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>396–397</span>, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1016/0042-6822%2859%2990043-1">10.1016/0042-6822(59)90043-1</a></span> (<a rel="nofollow" class="external text" href="https://www.sciencedirect.com/science/article/pii/0042682259900431">sciencedirect.com</a> [abgerufen am 28. Dezember 2021]).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rfr_id=info:sid/de.wikipedia.org:Dulbecco%E2%80%99s+Modified+Eagle%E2%80%99s+Medium&rft.atitle=Plaque+production+by+the+polyoma+virus&rft.au=R.+Dulbecco%2C+G.+Freeman&rft.date=1959-07-01&rft.doi=10.1016%2F0042-6822%2859%2990043-1&rft.genre=journal&rft.issn=0042-6822&rft.issue=3&rft.jtitle=Virology&rft.pages=396-397&rft.volume=8" style="display:none"> </span></span>

</li>

<li id="cite_note-2"><span class="mw-cite-backlink"><a href="#cite_ref-2">↑</a></span> <span class="reference-text">Eintrag zu <span style="font-style:italic;"><a rel="nofollow" class="external text" href="https://www.thermofisher.com/order/catalog/product/21010046?SID=srch-srp-21010046">Basal Medium Eagle (BME)</a></span> bei <i><a href="Thermo_Fisher_Scientific" title="Thermo Fisher Scientific">Thermo Fisher Scientific</a></i>, abgerufen am 19. September 2023.<span class="editoronly" style="display:none;"></span></span>

</li>

<li id="cite_note-3"><span class="mw-cite-backlink"><a href="#cite_ref-3">↑</a></span> <span class="reference-text">Eintrag zu <span style="font-style:italic;"><a rel="nofollow" class="external text" href="https://www.thermofisher.com/order/catalog/product/11095098?SID=srch-srp-11095098">MEM</a></span> bei <i><a href="Thermo_Fisher_Scientific" title="Thermo Fisher Scientific">Thermo Fisher Scientific</a></i>, abgerufen am 19. September 2023.<span class="editoronly" style="display:none;"></span></span>

</li>

<li id="cite_note-4"><span class="mw-cite-backlink"><a href="#cite_ref-4">↑</a></span> <span class="reference-text">Eintrag zu <span style="font-style:italic;"><a rel="nofollow" class="external text" href="https://www.thermofisher.com/order/catalog/product/12561049?SID=srch-srp-12561049">MEM α, no nucleosides</a></span> bei <i><a href="Thermo_Fisher_Scientific" title="Thermo Fisher Scientific">Thermo Fisher Scientific</a></i>, abgerufen am 19. September 2023.<span class="editoronly" style="display:none;"></span></span>

</li>

<li id="cite_note-5"><span class="mw-cite-backlink"><a href="#cite_ref-5">↑</a></span> <span class="reference-text"><span class="cite"><a rel="nofollow" class="external text" href="https://www.thermofisher.com/ch/en/home/technical-resources/media-formulation.48.html"><i>11885 - DMEM, low glucose, pyruvate (Formulation).</i></a> In: <i>Thermo Fisher Scientific.</i><span class="Abrufdatum"> Abgerufen am 28. Dezember 2021</span> (englisch).</span><span style="display: none;" class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2Fde.wikipedia.org%3ADulbecco%E2%80%99s+Modified+Eagle%E2%80%99s+Medium&rft.title=11885+-+DMEM%2C+low+glucose%2C+pyruvate+%28Formulation%29&rft.description=11885+-+DMEM%2C+low+glucose%2C+pyruvate+%28Formulation%29&rft.identifier=&rft.date=&rft.language=en"> </span></span>

</li>

</li>

</ol></div><div><div class="zim-footer">

Dieser Artikel wurde von <a class="external text" title="Zuletzt bearbeitet am 2023-09-19" href="https://de.wikipedia.org/wiki/?title=Dulbecco%E2%80%99s_Modified_Eagle%E2%80%99s_Medium&oldid=237466122">Wikipedia</a> herausgegeben. Der Text ist unter <a class="external text" href="https://creativecommons.org/licenses/by-sa/4.0/deed.de">Creative Commons Attribution-Share Alike 4.0</a> verfügbar, sofern nicht anders angegeben. Für die Mediendateien können zusätzliche Bedingungen gelten.

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